Vol. 8, Issue 5 (2019)

Author(s):
Snehal S Manekar and Manoj S Charde

Abstract:
The aim of present study is to evaluate the antiasthmatic potential of aerial parts of plant of Lawsonia inermis Linn. Using several experimental models. The dried and powdered aerial parts of Lawsonia inermis was extracted with continuous soxhlet extraction with Petroleum ether (40-60⁰ C), Chloroform, Ethyl acetate, Acetone, Methanol and Hydroalcoholic solvents. Preliminary phytochemical screenings of all extracts were done. Preliminary animal studies by In-vitro isolated goat trachea chain preparation of all extracts were done to find out potent extract. In this study, the methanolic extract of aerial parts of Lawsonia inermis was found to be potent comparative to other extract. The results of carrageenan induced rat paw oedema model indicated the dose dependant anti-inflammatory activity. As compared to standard drug (Indomethacin), methanolic extract showed similar activity which were found to be statistically significant (P<0.0001). The extent of DPPH radical scavenging was determined by calculated the IC₅₀ value of methanolic extract Lawsonia inermis (101.8) compared with the Ascorbic acid (114.7) taken as standard. In the present study the histamine induced dose dependent contraction of goat tracheal chain was significantly inhibited (p< 0.001) by methanolic extract of aerial parts of Lawsonia inermis (200 μg/ml). Thus the present study revealed that the methanolic extract of Lawsonia inermis (MEBP) has significant antihistaminic (H1 receptor antagonist) activity. In view the fact that tribal have well experience the antiasthmatic effects of the roots of Lawsonia inermis Linn. The results of our study, for the first time, show that the methanolic extract of aerial parts of Lawsonia inermis Linn. Possesses antioxidant, anti-inflammatory, Bronchodilator properties and therefore can be used for the antiasthmatic treatment.