Vol. 8, Issue 4 (2019)

Author(s):
Harshad B Patel, Urvesh D Patel, Chirag M Modi, Punit R Bhatt and Kajal B Pandya

Abstract:
An in vitro anti-diabetic activity of various indigenous medicinal plants was evaluated by α-amylase and α-glucosidase inhibition assay methods. Various extracts like chloroform, methanol and water extracts have been prepared by simple maceration of defatted plant powder using n-hexane. Qualitative phytochemical analysis was performed to evaluate the presence of active ingredients like flavonoids, alkaloids, glycosides, saponins, steroids, carbohydrates and tannins. Chloroform extracts of Gymnema sylvestre (Retz.) R.Br. ex Sm leaf, Lepidium sativum L. seed and Pueraria tuberosa (Willd.) DC. Tuber exhibited significantly higher (p < 0.05) inhibition of α-amylase activity as compare to standard drug acarbose. Water extracts of Solanum xanthocarpum Schrad. & H. Wendl. Leaf and Centratherum anthelminticum (L.) Gamble seed shown significantly higher (p < 0.05) inhibition of α-glucosidase followed by methanolic extracts of Pueraria tuberosa (Willd.) DC. Tuber and Moringa oleifera Lam. leaf as compared to acarbose. However, Solanum xanthocarpum Schrad. & H. Wendl. Leaf, Centratherum anthelminticum (L.) Gamble seed and Moringa oleifera Lam. leaf have shown quite lower activity against α-amylase enzyme. In conclusion, these extracts have shown the presence of active phytochemicals viz. flavonoids, alkaloids, glycosides, saponins and tannins, and showed significant in vitro anti-diabetic activity by inhibition of -amylase and -glucosidase enzyme activity.