Vol. 8, Issue 3 (2019)

Author(s):
P Rakshitha, RS Abhilash, MO Kurien, J Metilda, MD Pratheesh, MM Revathy

Abstract:
Successful oocyte recovery technique which yields higher quality and quantity of cumulus oocyte complexes play a key role for success in in vitro embryo production. In the present study a total of 231 abattoir ovaries were obtained from local slaughter house. COCs were retrieved from visible surface follicle of 2-8mm diameter by aspiration technique using oocyte collection medium. Mean ± S.E of number of follicles observed, number of oocytes recovered per ovary and recovery rate of oocytes were 5.58 ± 0.23, 4.83 ± 0.23 and 86.44 per cent. The mean yield of grade A, B, C and D oocytes obtained in the present study were 38.71 ± 2.15, 39.56 ± 1.49, 17.33 ± 1.62 and 4.59 ± 0.87. The yield of culture grade oocytes in the present study by aspiration technique was 78.27±2.08. The result indicates that aspiration technique is an efficient method to retrieve good quality COCs from crossbred cattle of Kerala.