Vol. 8, Issue 2 (2019)

Author(s):
Pallavi and Padma Singh

Abstract:
Present study based on the search of alternative and active compounds from Actinobacteria that can be used as a bio preservative. Since Actinobacteria are more potent antimicrobial agent which has the potential to replace antimicrobial chemical additives. During the present study, 25 different Actinobacteria isolated from the different soil samples such as from Ganga river bed, Banana and Sugarcane soil from different localities of Haridwar and its adjoining places. These cultures were screened for antimicrobial activity against various food borne microorganisms including Staphylococcus sp., Bacillus sp., Escherichia coli and Pseudomonas sp. isolated from various food products in order to identify potential antibiotic producers. Among these isolates, 12 showed antimicrobial activity against test microorganisms and 6 (AC9, AC14, R3, R4, R8 & R10) exhibited promising broad spectrum activity with zone of inhibition more than 15mm against all the tested organisms. These cultures were further identified on the basis of cultural, morphological and biochemical characteristics. Further studies on the bioactive metabolites from these cultures which will be useful for discovering novel compounds of clinical industrial significance.