Vol. 8, Issue 1 (2019)

Author(s):
A Ranjini and HK Manonmani

Abstract:
Resveratrol has been regarded to exhibit antigenotoxic effects. Resveratrol was isolated from healthy grape berries and wine. The trans-resveratrol was purified, characterized by HPLC and LC-MS. In this study the protective effect of trans-resveratrol against genotoxicity induced by acrylamide (AA), a toxicant formed in carbohydrate-rich foods subjected to temperatures, above 140º C and its metabolite glycidamide (GA) resulting from cytochrome P450 2E1 (CYP2E1) was investigated in HepG2 cells. Pretreatment of HepG2 cells with resveratrol (RES) showed ameliorating effects on AA and GA induced cytotoxicity, decreased the reactive oxygen species (ROS), and prevented DNA damage, micronucleus induction. Also resveratrol exhibited decreased in the activity, mRNA levels of CYP2E1 and increased activity and gene expression of GST, thus preventing the accumulation of GA. Also, RES showed the upregulation of few of the DNA repair genes thus preventing mutation accumulation induced by AA and GA. In conclusion RES potential antioxidant property protects HepG2 cells from genotoxicity induced by AA and GA.