Vol. 5, Issue 8 (2016)

Author(s):
Nasreen Begum, Srisailam Keshetti and Uma Maheshwara Rao Vattikuti

Abstract:
The study was conducted to investigate in vitro anti-inflammatory and COX-2 inhibitory activities of dried leaves of Origanum vulgare. For the present investigation, 300 µg / ml and 500 µg / ml of petroleum ether, chloroform, ethyl acetate, methanol and water extracts were used. The extracts were obtained by successive soxhlet extraction. In vitro anti-inflammatory activity was studied by membrane stabilization of HRBCs. The percentage of membrane stabilization was compared with standard drug Diclofenac sodium at a concentration of 300 µg / ml and 500 µg / ml. In vitro COX-2 inhibitory activity was evaluated by enzyme immunoassay. Methanolic extract at a concentration of 500 µg / ml showed significant membrane stabilization of 84.34% and 74.52% of remarkable COX-2 inhibition. The results observed thus, suggest that the methanolic extract of dried leaves of Origanum vulgare, possesses promising in vitro anti-inflammatory and COX-2 inhibitory activity.