Vol. 5, Issue 3 (2016)

Author(s):
Rohith Singh Tahkur, Geeta Soren, Rama Mohan Pathapati, Madhavulu Buchineni

Abstract:
Background: Moringa Olifera commonly known as drumstick belongs to the Moringaceae family. A number of medicinal properties attributed to different parts of M. Olifera. we evaluated diuretic activity of alcoholic extract of Moringa oleifera leaves in swiss albino rats compared with hydrochlorothaizide. Methods: In first group, six albino rats was kept as control, was given only 0.9% normal saline 25ml/kg body weight orally. Another group of 6 rats were fed with normal saline 25ml/kg along with standard hydrochlorothiazide 2.5mg/kg. The Third, fourth and fifth groups of 6 rats each were taken as test group and the crude extract of Moringa Oleifera which was obtained in liquid form along with normal saline was given, keeping the volume constant, in doses of 50,100 and 200mg/kg bodyweight. Metabolism cage was used to collect urine in beakers for a period of 5 hours and 24 hours. Analysis of the data was done using ANOVA and Tuckey test. P values of less than 0.05 were considered significant. Results: After 5 hours of urine analysis-The urinary volume of the control group was 7.3±0.2 mL and the urinary excretion of Na⁺, K⁺ and Cl^- are 77.7±1.2 mEq/L, 21±1 mEq/L and 262.2±0.5 mEq/L respectively. The urinary volume of the standard group was 13.37±0.95 mL and the urinary excretion of Na⁺, K⁺ and Cl^- are 168.4±3.39 mEq/L, 16±0.62 mEq/L and 147.46±5.79 mEq/L. After 24 hours of urine analysis-The urinary volume of the control group was 13.7±0.5 mL and the urinary excretion of Na⁺,K⁺ and Cl^- are 63.72±0.56, 22.05±0.34 and 265.5±1 mEq/L respectively. The urinary volume of the Standard group was 4.13±0.73 mL and the urinary excretion of Na⁺,K⁺ and Cl^- are 173±45, 20.01±0.15 and 151.41±6.52 mEq/L Conclusion: Moringa oleifera leaves extract produced dose dependant diuretic action which is greater than control lesser than hydrochloro thiazide. The extract showed dose dependent saluretic effect. On the other hand, potassium sparing activity was not observed.