Vol. 4, Issue 6 (2015)

Author(s):
Jansi rani L, Venkatesh B, Kumaran T

Abstract:
Sericulture is an agro-based income generative venture. Developing countries, the loss due to diseases in sericulture is to the tune at 10% of the total crop loss. In the present study the diseased silkworm were collected, and immobilized by chloroform and surface sterilized by methanol. The larvae were dissected under aseptic condition to remove foregut, silkgand, reproductive system and a piece of skin. Each observed colony was purified and subjected to morphological, physiological and biochemical tests. As a result the organisms identified were Staphylococuss aureus, Staphylococcus epidermis, Staphylococcus albus, Bacillus thuriensis and Bacillus Orpheus. Sentivity to antibiotics was tested for selected pathogens isolated from disease infected worms. The antibiotics viz. streptomycin, ampicillin and penicillin were used. Control was maintained. In vitro screening of the pathogen for antibiotic sensitivity revealed differential sensitivity microbes for different antibiotics. Streptomycin was found to be the most effective antibiotics to control the pathogen.