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Vol. 3, Issue 11 (2015)

Second derivative spectrophotometric method development and validation for simultaneous estimation of gatifloxacin and prednisolone acetate in their combined dosage form

Author(s):
Prasanna Kumar Pradhan, Naimish Raiyani, Shreya R. Shah, Grishma H. Patel, Umesh Upadhyay
Abstract:
Gatifloxacin and Prednisolone Acetate combination is used for prevention and treatment of ophthalmic bacterial infections and inflammatory conditions. In Second Derivative Spectrophotometric method, Methanol is used as a mobile phase. linearity range obtained for the method were 1.5 – 4.5 μg/ml and 5 - 15 μg/ml with corresponding correlation coefficient of 0.9990 and 0.9994, for Gatifloxacin and Prednisolone Acetate respectively. Detection of Gatifloxacin and Prednisolone Acetate at 268 nm and 243 nm respectively. The method was found to be rapid, accurate and precise. This method was validated according to ICH guidelines.
Chemical structure of Gatifloxacin
Fig.: Chemical structure of Gatifloxacin
Pages: 06-10  |  655 Views  10 Downloads
How to cite this article:
Prasanna Kumar Pradhan, Naimish Raiyani, Shreya R. Shah, Grishma H. Patel, Umesh Upadhyay. Second derivative spectrophotometric method development and validation for simultaneous estimation of gatifloxacin and prednisolone acetate in their combined dosage form. 2015; 3(11): 06-10.
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