Volume 3, Issue 7


Pancreatic lipase inhibitory screening of Citrullus lanatus leaves

Author: Ajithadas Aruna, Vijayalakshmi K, Karthikeyan V

Abstract: Natural remedies offer an exciting opportunity and promise for the development of new therapeutic lead molecule to the treatment of obesity by inhibiting the digestion and absorption of dietary lipids which is valuable alternative to synthetic one. In this study to evaluate pancreatic lipase inhibitory activity of Methanolic extract of Citrullus lanatus (MECL) leaves.
Preliminary phytochemical screening of MECL was done by standard procedure. Total Phenolic and Flavonoid content, Tannin content and estimation of Vitamin C were estimated by using Gallic acid, Quercetin, tannic acid and standard Ascorbic acid calibration curve respectively. Pancreatic lipase (PL) was done by using chicken pancreas and orlistat used as a standard.
Preliminary phytochemical screening showed the presence of carbohydrates, triterpenoids, proteins, alkaloids, tannins, saponins, flavonoids, sterols and absence of glycosides, volatile and fixed oil. Phenolic content (in terms mg GAE/g of extract), Flavonoids (mg Quercetin equivalent/g of extract) and Tannin content (mg Tannic acid/g of extract) present in the MECL were found to be 47.05±0.338, 89.99±0.30 and 290.9 ± 0.12 mg/g respectively. The amount of Vitamin C of MECL was found to be 34.00 ± 0.009mg/g. Percentage inhibition and IC50 of MECL and orlistat was found to be 61.77 ± 1.102 & 73.63 ± 0.553 at a concentration of 5mg/ml and 3.962 mg/ml & 3.420 mg/ml respectively.

The dose dependent pancreatic lipase inhibitory activity was observed. The extract of Citrullus lanatus possesses a good inhibitory activity on pancreatic lipase.It may be the presence of flavanoid, tannins, terpenes, phenols, vitamin C and saponins. MECL employed as a therapy for the management of the obesity epidemic.


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