Volume 2, Issue 3
UV Spectrophotometric Absorption Correction Method for the Simultaneous Estimation of Efavirenz, Lamivudine and Zidovudine in Tablet Dosage Forms
Authors: Yadavalli Rekha*, Yellina Haribabu, Sheeja Velayudhankutty, Sosamma Cicy Eapen, Jane Mary.
Grace College Of Pharmacy, Kodunthirappully, Palakkad-678004, Kerala, India.
Abstract: The present paper describes simple, accurate, rapid, precise and sensitive UV spectrophotometric absorption correction method for the simultaneous determination of Efavirenz, Lamivudine and Zidovudine in combined tablet dosage form. Methanol was used as solvent. The wavelengths selected for the analysis using absorption correction method were 305 nm, 250 nm and 254 nm for estimation of Efavirenz, Lamivudine and Zidovudine, respectively. Beer’s law obeyed in the concentration range of 4-12 μg/mL, 1-3 μg/mL and 2-6 μg/mL for Efavirenz, Lamivudine and Zidovudine, respectively. The mean percentage drug content for Efavirenz, Lamivudine and Zidovudine were found to be 100%, 99.03% and 98.66%, respectively and the % RSD value was found to be less than 2 which shows the precision of method. The developed method was validated statistically and by recovery studies. The high recovery and low coefficients of variation conforms the suitability of the method for simultaneous analysis of three drugs in combined tablets. Statistical analysis proves that the method was found to be suitable for the routine quality control analysis of Efavirenz, Lamivudine and Zidovudine in pure and pharmaceutical dosage forms.
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