Vol. 12, Special Issue 9 (2023)

Author(s):
Yogesh, Santhosh Gaikwad, Gururaj VK and Kalicharan Nayal

Abstract:
The present research work was conducted on “Gross anatomical and histomorphological studies on testis of dog (Canis domesticus)”. The testis of dogs collected from 6 non-descript, healthy adult dogs at sterilization center BSPCA, Parel, Mumbai. The normal structure of a dog's testis was investigated by using sections stained with hematoxylin and eosin, Van Gieson's stain for collagen fibres, Weigert's technique for elastic fibres and Wilders stain for reticular fibres. While periodic Acid Schiffs techniques for glycogen, Gomori's for alkaline phosphatase for alkaline phosphatase, and Gomori's modified lead Nitrate method for acid phosphatase were determined. PAS staining reaction revealed that the glycogen (mucopolysaccharides) was present in the various component of testis from intense to moderates. The alkaline phosphatase and acid phosphatase activity were also been observed from moderate to mild form in various component of testis.