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Vol. 12, Special Issue 9 (2023)

Purification of Trypanosoma evansi from mice blood by anion exchange (Diethylaminoethyl cellulose) column chromatography

Author(s):
M Fibi Rani, GS Sreenivasamurthy, M Udaya Kumar and P Kalyani
Abstract:
In the current study, a dog strain of T. evansi was in-vivo passaged in Swiss albino mouse for propagation. 3 DPI, parasitaemia was confirmed by examination of wet blood film of the mouse. Pure and viable Trypanosoma evansi were separated from the mouse blood by anion exchange chromatography using the DEAE cellulose minicolumn and were counted using Neubauer’s haemocytometer. This is the best method for the purification of T. evansi that permits further serological and molecular research investigations.
Pages: 364-368  |  178 Views  99 Downloads
How to cite this article:
M Fibi Rani, GS Sreenivasamurthy, M Udaya Kumar and P Kalyani. Purification of Trypanosoma evansi from mice blood by anion exchange (Diethylaminoethyl cellulose) column chromatography. The Pharma Innovation Journal. 2023; 12(9S): 364-368.

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