Vol. 12, Special Issue 9 (2023)

Author(s):
M Fibi Rani, GS Sreenivasamurthy, M Udaya Kumar and P Kalyani

Abstract:
In the current study, a dog strain of T. evansi was in-vivo passaged in Swiss albino mouse for propagation. 3 DPI, parasitaemia was confirmed by examination of wet blood film of the mouse. Pure and viable Trypanosoma evansi were separated from the mouse blood by anion exchange chromatography using the DEAE cellulose minicolumn and were counted using Neubauer’s haemocytometer. This is the best method for the purification of T. evansi that permits further serological and molecular research investigations.