Abstract:The present study was carried out to analyze the various milk constituents of Luit buffalo of Assam. A total of 172 milk samples were randomly collected from farm (ICAR-Network project on Buffalo, Assam Agricultural University, Khanapara, Guwahati, Assam, India) as well as field conditions (Dhemaji, North Lakhimpur and Sivasagar districts of Assam, India). Milk Analyzer, LAKTAN 1-4 (Model: 220) was utilized to measure different milk constituents. Statistical analysis of data was carried out in the Department of Animal Genetics and Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-22. The overall least-squares means (µ) along with standard error (SE) for percentage of milk fat, solid-not fat, milk protein and total solids were found to be 7.591±1.148, 9.047±0.052, 3.032±1.126 and 16.638±1.125, respectively. Effect of location was found to have significant influence (p˂0.05) on milk fat percentage, protein percentage and total solid percentage.