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Vol. 12, Special Issue 8 (2023)

PCR assay for the detection of partial variable surface glycoprotein gene in Trypanosoma evansi in canines

Author(s):
M Fibi Rani, GS Sreenivasamurthy, M Udaya Kumar and P Kalyani
Abstract:
The Polymerase Chain Reaction assay using the species-specific diagnostic RoTat 1.2 VSG primers targeting the partial VSG gene of T. evansi was standardized, which amplified a product of 205 bp length. The standardized PCR assay showed an analytical sensitivity of detection up to 1 pg DNA of T. evansi in our lab. A total of 314 blood samples were collected from dogs and 7.64% (24/314) were found positive by standardized PCR assay in and around Hyderabad, Telangana. A non-significant difference existed in the prevalence in male (7.58%) and female (7.69%) dogs. Age wise comparison of prevalence indicated a significantly higher rate of infection in 1-8 years (11.46%), > 8 years (5.88%), and < 1 year (1.38%) age dogs in descending order. The highest prevalence was noticed among Mongrels (15.68%) and then crossbred dogs (13.04%), Labradors (3.92%), German shepherd dogs (3.22%), Doberman (2.94%), Pomeranian (2.56%) in descending order and no prevalence was recorded in Saint Bernard, Pug and Golden Retriever.
Pages: 1350-1353  |  152 Views  74 Downloads
How to cite this article:
M Fibi Rani, GS Sreenivasamurthy, M Udaya Kumar and P Kalyani. PCR assay for the detection of partial variable surface glycoprotein gene in Trypanosoma evansi in canines. The Pharma Innovation Journal. 2023; 12(8S): 1350-1353.

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