Vol. 12, Special Issue 7 (2023)

Author(s):
A Shirisha, A Vijaya Kumar, Laxman Chattlod, M Shashi Kumar, Raghupathi and N Krishnaiah

Abstract:
The present study mainly deals with the green synthesis, characterization and evaluation of antibacterial properties of silver nanoparticles (AgNPs) synthesized by using the leaf extract of Moringa oleifera and fruit extract of Tamarindus indica. In this research, three distinct ratios of 1 mM silver nitrate to Moringa oleifera leaf extract 95:5, 90:10, and 85:15 were used to synthesize silver nanoparticles. The 90:10 ratio was chosen for further investigation based on having the largest peak, a decent size, and stability. To a silver nitrate solution, tamarind fruit extract was added until the solution's color changed from light brown to chocolate brown. The UV-Visible spectroscopy, Zeta potential, and size distribution by intensity were used to characterise the produced silver nanoparticles. The surface plasmon absorption band with maximal values at 420 nm and 430 nm respectively, indicated the existence of silver nanoparticles in the silver nano solution made with Moringa oleifera and Tamarindus indica fruit extract. Silver nanoparticles with sizes of 110.2 nm and 130.2 nm were produced from fruit extracts of Moringa oleifera and Tamarindus indica. Their zeta values were -12.5 mV and -15.5 Mv respectively. The agar well diffusion method was used to test the antibacterial effectiveness of nanosilver, and the silver nanoparticles demonstrated strong antibacterial activity against Staphylococcus aureus.