Vol. 11, Special Issue 9 (2022)

Author(s):
Bhanu Brijlal Khutey, Raju Sharda, Rukmani Dewangan, SK Tiwari, Sumeet Pal and MV Kamble

Abstract:
The present study was conducted on 18 healthy dogs of either sex weighing between 10-20 kg to evaluate the alternations on haemato-biochemical parameters following administration of butorphanol, dexmedetomidine and acepromazine as premedicants in combination with thiopentone sodium for inducing general anaesthesia. The experimental work was divided into three groups BT (butorphanol-thiopentone sodium), DT (dexmedetomidine-thiopentone sodium) and AT (acepromazine-thiopentone sodium). Glycopyrrolate @ 0.02 mg/ kg intramuscularly was administered 10 minutes prior to treatment in all the groups of animals. The animals of group BT, DT and AT were premedicated intramuscularly with butorphanol @ 0.3 mg/kg b.wt. Dexmedetomidine @ 10 µg/kg b.wt. And acepromazine @ 0.4 mg/kg b.wt. Respectively and general anaesthesia was induced with thiopentone @ 18 mg/kg b.wt. Intravenously. Haematological parameters viz. Hb, PCV, TEC, TLC and DLC and biochemical parameters viz. serum glucose, serum total protein, serum urea nitrogen, serum creatinine, ALT and AST were estimated before sedation (0) and at 30, 60,120 min. and 6 hrs post thiopentone anaesthesia. Haematological studies revealed non-significant changes in haemoglobin, packed cell volume, total erythrocyte count and total leucocyte count following thiopentone anaesthesia at various time intervals in all the groups. There was significant (p<0.05) increase in neutrophils with a consequent non-significant decrease in lymphocyte values in all the three groups. Serum glucose showed a significant (P<0.01) increase in all the groups initially which returned to base values by 6 hrs. Other biochemical parameters like serum total protein, serum urea nitrogen, serum creatinine and serum enzymes viz. AST and ALT values showed non-significant changes at various time intervals in all the three groups but were within normal range. It was concluded that thiopentone sodium in combination with butorphanol, dexmedetomidine and acepromazine does not produce any harmful effect on vital organs and changes remained within physiological limits, thus combinations can be safely used as a general anaesthetic in canines.