Vol. 11, Special Issue 9 (2022)

Author(s):
Vanitha Sree K, Usha Rani M, Gopala Reddy A, Lakshman M, Kalyani P, Anilkumar Banothu, Rajender B and Venkata Rao KV

Abstract:
This study was conducted to know the therapeutic efficacy of Galangin and Piperine individually and in combination with the Bleomycin-induced oxidative stress. Fifty C57BL/6 male mice of 6-7 weeks of age weighing ~28-30 g were divided into seven groups of six animals each. Group 1 served as normal control; Group 2 served as disease Control -Bleomycin sulfate [BLM] @ 1.5 IU/ Kg bwt, oropharyngeal route, Single dose on 0^th day; Group 3 treated with Galangin @ 5 mg/kg bwt/orally/daily per se; Group 4 is Piperine @ 50 mg/kg bwt/orally/daily per se; Group 5 treated Bleomycin sulfate + Galangin @ 5 mg/kg bwt/orally/daily; Group 6 Bleomycin sulfate + Piperine @ 50 mg/kg bwt/orally/daily and Group 7 Bleomycin sulfate + Galangin @ 2.5 mg/kg bwt/orally/daily + Piperine @ 25 mg/kg bwt/orally/daily. Blood samples were collected at the end of the experiment to evaluate hematological parameters body weights were measured on the 7th, 14th, and 21st day. The study results of treatment groups (5, 6 and 7) have revealed a significant (p>0.05) increase in body weights compared to group 2 at different time intervals. Similarly, TLC was significantly higher, and Hemoglobin and Total erythrocyte count were lower in group 2 compared to treatment groups 5, 6 and 7. In conclusion, among treatment groups, the combination groups (7) had shown a better ameliorative effect than the individually treated group.