A study on phytochemical screening and antidiabetic potential of Vitex negundo leaf extract in rats
Sonam Sharma, AH Ahmad, Disha Pant, Munish Batra, PC Patwal, Nidhi Arya and Deeksha Maletha
Objective: The main objective of this study was to perform the phytochemical analysis of the hydroethanolic extract of leaves of Vitex negundo (HEVN) and to assess its antidiabetic potential following oral administration @ 100 mg/kg and 200 mg/kg B.WT for 28 days in Streptozotocin induced diabetic rats. For the in-vivo study, forty-two rats of 2-2.5 months of age were divided randomly into seven groups with six rats in each group. Group I served as normal control group. Group II, III, VI and VII, received a single intraperitoneal injection of Streptozotocin @ 45 mg/kg B.WT to induce diabetes. Rats showing blood glucose level greater than 200 mg/dl on 7th day were considered hyperglycemic and chosen for the experiment. Group II represented as diabetic control. Group III (diabetic) was treated with glibenclamide @ 1 mg/kg B.WT. Orally for 28 days. Group IV and V (non-diabetic) were given HEVN orally @100 mg/kg B.WT and 200 mg/kg B.WT, respectively, for 28days. Group VI and VII (diabetic) were given HEVN orally @100 mg/kg B.WT and 200 mg/kg B.WT, respectively, for 28 days. The phytochemical screening of hen was also performed to detect the presence of phytoconstituents. The qualitative phytochemical analysis of HEVN revealed the presence of alkaloids, flavonoids, tannins, terpenoids, saponins, glycosides, phenols and fixed oils and fats. The quantitative analysis of HEVN revealed total phenolic content to be 390.74±3.60 mg Gallic acid equivalent (GAE) per gm. of the dry weight of extract and total flavonoid content to be 291.00±3.42 mg rutin per gm. of the dry weight of extract. The results of the in-vivo study showed that Streptozotocin administration caused significant (p < 0.05) elevation in blood glucose and HbA1c level in rats which was restored back towards normal in HEVN treated groups in a dose-dependent manner. On histopathological examination, group II showed histopathological disintegrate in pancreas which was reverted back towards normal in HEVN treated rats @ 200 mg/kg B.WT. Thus, the hydroethanolic extract of leaves of Vitex negundo (HEVN) @ 100 mg/kg and 200 mg/kg has ameliorative potential against Streptozotocin (@ 45 mg/kg) induced diabetes in rats in a dose-dependent manner.
How to cite this article:
Sonam Sharma, AH Ahmad, Disha Pant, Munish Batra, PC Patwal, Nidhi Arya and Deeksha Maletha. A study on phytochemical screening and antidiabetic potential of Vitex negundo leaf extract in rats. The Pharma Innovation Journal. 2022; 11(9S): 254-259.