Toll Free Helpline (India): 1800 1234 070

Rest of World: +91-9810852116

Free Publication Certificate

Vol. 11, Special Issue 11 (2022)

Cytochrome P4501A responses in Carassius carassius in relation to nitrate and phosphate levels of Wular Lake of Jammu and Kashmir

Author(s):
Saima Andleeb, Irfan Ahmad, Asim Iqbal Bazaz, Oyas Asimi, Bilal Ahmad Bhat, Feroz Ahmad Shah and Gohar Bilal Wani
Abstract:
Hepatic CYP1A induction in fish due to specific groups of pollutants has been proposed as an early warning system and most sensitive biological response to environmental contamination levels. Fish exposure induces the expression of the CYP1A gene through receptor-mediated mechanisms. The objective of this study was to detect Cytochrome P4501A in Carassius in relation to nitrate and phosphate levels of the Wular Lake. The samples (fishes and water) were collected on monthly basis from three different sites of the lake. CYP1A was detected in all the samples examined throughout the study period using Enzyme Linked Immuno Sorbant Assay (ELISA). Using one way analysis of variance, p<0.01 was obtained which indicated that there was a significant difference (p<0.05) in CYP1A levels among the 4 sampling sites viz., Kulhama, Laherwalpora and Ashtung and control group. There was the +ve significant correlation (r = 0.94, p<0.01) between total phosphate and nitrate. Further, it was observed that CYP1A showed the +ve correlation with both phosphate (r= 0.89, p<0.01) as well as nitrate concentrations (r=0.88, p<0.01). In the present study, CYP1A concentration was studied in relation to the total phosphorous and nitrate levels at the respective sites. Both CYP1A level as well as the phosphate and nitrate levels was highest in samples collected from Site B (Laherwalpora) and lowest in the samples collected from control group. It was evident from the present study that with the increase in phosphate and nitrate content, CYP1A levels also increased considerably. In other words the two parameters i,e phosphate and nitrate concentration in water showed a direct relationship with CYP1A concentration in fishes.
Pages: 2631-2636  |  158 Views  51 Downloads
How to cite this article:
Saima Andleeb, Irfan Ahmad, Asim Iqbal Bazaz, Oyas Asimi, Bilal Ahmad Bhat, Feroz Ahmad Shah and Gohar Bilal Wani. Cytochrome P4501A responses in Carassius carassius in relation to nitrate and phosphate levels of Wular Lake of Jammu and Kashmir. The Pharma Innovation Journal. 2022; 11(11S): 2631-2636.

Call for book chapter