Vol. 11, Special Issue 11 (2022)

Author(s):
Khushbu Rana, Bharat Bhanderi and Seema Gadhavi

Abstract:
Contagious ecthyma is an acute, highly contagious, debilitating and economically important viral skin disease, commonly occurring in sheep and goats. Contagious ecthyma is light to moderate and self-limiting disease, but its economic impact can be very significant. The disease is zoonotic in nature. In the present study, during the period from June-2021 to December-2021, a total of 30 samples of affected goats were collected. The different genes like B2L, VIR, GIF, viral RNA polymerase and A32L were targeted for molecular detection of ORFV. Molecular detection of ORFV by targeting B2L gene revealed 100% (30/30) positive result by semi-nested PCR. The positivity of ORFV by targeting VIR, RNA polymerase and A32L was 93.33% (28/30). A total 29 (96.67%) goats’ scab samples turned positive, while only one (3.33%) sample from Goat 5 was found negative in GIF gene based PCR.