Vol. 11, Special Issue 11 (2022)

Materials and Methods: The current research was carried out on 45 Pati ducks from Assam. The ducks were procured from Pathsala and nearby areas of Bajali district, Assam. The Pati duck were brought to the Department of Anatomy and Histology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-22, where they were sacrificed using Gracy’s procedure (1986). The birds were placed on a clean dissecting table after slaughter, and the skin and fascia were gently reflected without disturbing the organs. By making a ventro-median incision, the abdominal cavity of each Pati duck was revealed, and the abdominal muscle layers, peritoneum, and air sacs of the abdominal region were properly reflected. Then collected the parts of lymph node, Fabricius' bursa, and gut with lymphoid tissue or Peyer's patches. After that, the samples were frozen in liquid nitrogen (-196 °C). Cryosections were cut at a thickness of 10 µm and stored at -20 °C for a period of time at -20 °C. The following processes were used to prepare them for histochemical staining:

a. Lead method for ATPase (Bancroft, 2008)

b. 1 Naphthyl acetate method for non-specific esterase (Bancroft, 2008)

Results: The lymphatic nodule of the lymph node had a significant reaction, while the interfollicular area had a moderate reaction. B-lymphocytes were found in greater numbers in the lymphatic nodule of the lymph node than in the interfollicular area and capsule. T-lymphocytes were found in lower numbers in lymphatic nodules and were completely missing in lymph node capsules. In the follicular related epithelium and undifferentiated epithelial cell of the lymphoid follicle of Fabricius' bursa, there was a robust reactivity to ATPase. In Pati ducks, the lamina propria of Fabricius' bursa and the cortex of lymphoid follicle showed no reactivity to non-specific esterase, while the medulla of lymphoid follicle showed no sensitivity to enzyme. The reaction revealed that B-lymphocytes were more abundant in the cortex than in the medulla, and that T-lymphocytes were absent. The lymphoid follicles of both Peyer's patches and solitary lymphoid nodules revealed high adenosine triphosphatase activity. The lymphoid nodules of the gut in Pati ducks showed a high sensitivity to non-specific esterase activity. The presence of B-lymphocytes and T-lymphocytes was higher in lymphoid follicles of the gut in both reactions.

Conclusion: This study will helpful to the duck farmer, poultry scientist, physiologist and microbiologist for vaccine production and duck meat, egg production and diseases control regime.