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Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
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Vol. 11, Special Issue 11 (2022)
Assessing the antioxidant activity of peptides obtained from chicken intestine hydrolysate in canine pellet food using DPPH radical scavenging assay
Author(s):
E Vimalraj, R Ramani, V Appa Rao, M Parthiban, R Narendrababu, A Abinaya and S Arulkumar
E Vimalraj, R Ramani, V Appa Rao, M Parthiban, R Narendrababu, A Abinaya and S Arulkumar
Abstract:
Chicken intestine was hydrolysed by protease P food grade fungal enzyme. Chicken intestinal hydrolysate was ultrafiltered using 10 kda molecular weight cut-off and the filtrate is freeze dried. Then the low molecular weight peptides is incorporated in canine pellet feed at 5%, 6% and 7% levels. Canine pellet feed with different levels of incorporated peptides was assessed for antioxidant activity using DPPH radical scavenging assay. The mean±SE values of IC50 value for anti-oxidant activity of bioactive peptide incorporated at different levels of 5%, 6% and 7% in canine pet food were 1.80±0.4 mg/ml, 1.41±0.6 mg/ml and 0.20±0.70 mg/ml respectively.
Chicken intestine was hydrolysed by protease P food grade fungal enzyme. Chicken intestinal hydrolysate was ultrafiltered using 10 kda molecular weight cut-off and the filtrate is freeze dried. Then the low molecular weight peptides is incorporated in canine pellet feed at 5%, 6% and 7% levels. Canine pellet feed with different levels of incorporated peptides was assessed for antioxidant activity using DPPH radical scavenging assay. The mean±SE values of IC50 value for anti-oxidant activity of bioactive peptide incorporated at different levels of 5%, 6% and 7% in canine pet food were 1.80±0.4 mg/ml, 1.41±0.6 mg/ml and 0.20±0.70 mg/ml respectively.
These values were compared with the IC50 value for anti-oxidant activity of canine pet food prior to addition of bioactive peptides which was 1.84±0.4 mg/ml which is taken as standard. The test of significance revealed that there was a highly significant (p<0.01) difference between the IC50 values of canine pet food incorporated with different levels (5%, 6% and 7%) of bioactive peptides. The canine pet food with 7% incorporated bioactive peptide had lower IC50 value than the other levels indicating that the potency of the extracted peptides has been exhibited in the incorporated canine pet food.
Pages: 99-101 | 310 Views 102 Downloads
How to cite this article:
E Vimalraj, R Ramani, V Appa Rao, M Parthiban, R Narendrababu, A Abinaya and S Arulkumar. Assessing the antioxidant activity of peptides obtained from chicken intestine hydrolysate in canine pellet food using DPPH radical scavenging assay. The Pharma Innovation Journal. 2022; 11(11S): 99-101.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
