Assessing the antioxidant activity of peptides obtained from chicken intestine hydrolysate in canine pellet food using DPPH radical scavenging assay

E Vimalraj; R Ramani; V Appa Rao; M Parthiban; R Narendrababu; A Abinaya; S Arulkumar

Assessing the antioxidant activity of peptides obtained from chicken intestine hydrolysate in canine pellet food using DPPH radical scavenging assay

Author(s):
E Vimalraj, R Ramani, V Appa Rao, M Parthiban, R Narendrababu, A Abinaya and S Arulkumar

Abstract:
Chicken intestine was hydrolysed by protease P food grade fungal enzyme. Chicken intestinal hydrolysate was ultrafiltered using 10 kda molecular weight cut-off and the filtrate is freeze dried. Then the low molecular weight peptides is incorporated in canine pellet feed at 5%, 6% and 7% levels. Canine pellet feed with different levels of incorporated peptides was assessed for antioxidant activity using DPPH radical scavenging assay. The mean±SE values of IC₅₀value for anti-oxidant activity of bioactive peptide incorporated at different levels of 5%, 6% and 7% in canine pet food were 1.80±0.4 mg/ml, 1.41±0.6 mg/ml and 0.20±0.70 mg/ml respectively.

These values were compared with the IC₅₀ value for anti-oxidant activity of canine pet food prior to addition of bioactive peptides which was 1.84±0.4 mg/ml which is taken as standard. The test of significance revealed that there was a highly significant (p<0.01) difference between the IC₅₀ values of canine pet food incorporated with different levels (5%, 6% and 7%) of bioactive peptides. The canine pet food with 7% incorporated bioactive peptide had lower IC₅₀ value than the other levels indicating that the potency of the extracted peptides has been exhibited in the incorporated canine pet food.

Pages: 99-101 | 69 Views 4 Downloads

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Vol. 11, Special Issue 11 (2022)

Assessing the antioxidant activity of peptides obtained from chicken intestine hydrolysate in canine pellet food using DPPH radical scavenging assay

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