Vol. 10, Special Issue 7 (2021)

Author(s):
Shahziya Shah, Anayitullah Chesti, Mansoor Rather, Saima Manzoor, Rizwana Malik and Junaid Khan

Abstract:
In the modern high intensity aquaculture, probiotics offer an encouraging substitute to chemicals and antibiotics in aquatic animals, one such important application of probiotics is their use as Immune enhancer in aquaculture, in addition to growth and water quality management. So the present study was carried to evaluate the effect of dietary incorporation of probiotic - Bacillus subtilis on the hematology of grass carp (Ctenopharyngodon idella. The probiotic - Bacillus subtilis was mixed with the basal diet (Protein 32%) in three different concentrations (0.5, 1.0 and 1.5% designated as T₁, T₂, and T₃). The basal feed with no probiotic was used as control. The study was conducted for a period of 60 days. Feeding was done twice a day at the rate of 5% of their body weight. Hematological parameters (TLC and DLC) were also examined. The probiotic treated fish (T³, 1.5%/100g) displayed highest WBC count as compared to the control.