Vol. 10, Special Issue 7 (2021)

Author(s):
Thirtha Kumar AR, Kshama MA, Ramesh PT, Narayana Bhat M, Narayanaswamy HD, Rathnamma D and Mamatha GS

Abstract:
The present study was undertaken with the objectives of studying the occurrence of Theileria orientalis in cattle in and around Bengaluru by using Giemsa stained blood smear examination and Polymerase Chain Reaction (PCR) in cattle. Fifty clinical cases were screened based on history and clinical signs and blood was collected for a definitive diagnosis and evaluation of various hematological parameters. Diagnosis was made based on blood smear examination by using Giemsa stain and PCR. Of the fifty clinical cases, eighteen (36%) cases were positive for theileriosis through blood smear examination. On PCR, of the 50 samples, 34 (68%) were positive for theileria. Of the 34 positive samples, 26 (76.5%) were positive for T. orientalis by species specific primers.