Vol. 10, Special Issue 1 (2021)

Author(s):
Ashitha Suresh, MK Shukla, Dharmendra Kumar, OP Shrivastava and BC Sarkhel

Abstract:
The present experiment was undertaken to study oocyte retrieval from abattoir derived goat ovaries and to study their nuclear maturation for in vitro embryo production. A total of 348 abattoir derived goat ovaries were subjected to aspiration technique for oocyte retrieval resulting in recovery of 1035 cumulus oocyte complexes (COCs) from 1422 follicles with an average of 3.20±0.32 COCs per ovary and recovery rate of 73.93±1.49. The nuclear maturation rate of 89.78±0.50 was also observed in in vitro matured goat oocytes after staining with Hoechst 33342 dye.