Vol. 14, Issue 8 (2025)

Author(s):
Maria Rodrigues, Lukas Fischer and Jovana Petrović

Abstract:
The edible mushroom Pleurotus ostreatus is well recognized for its nutraceutical potential, bearing high-quality protein, bioactive polysaccharides (e.g. ?-glucans), antioxidants, vitamins, and minerals. Conventional cultivation substrates, typically straw or sawdust, may not optimally support the full biosynthesis and accumulation of these beneficial compounds. This research investigates the hypothesis that supplementing the growth substrate with biochar, derived from spent mushroom substrate (SMS) or other lignocellulosic waste, can improve the nutraceutical profile of P. ostreatus fruiting bodies without compromising yield. Experimental results demonstrated that biocharamended substrates produced mushrooms with significantly higher protein content, enhanced mineral accumulation (especially essential elements), and elevated antioxidant capacity compared to controls. Yield and biological efficiency remained within acceptable ranges, indicating the feasibility of this approach. These findings suggest that biochar supplementation offers a sustainable, low-cost, and ecofriendly strategy to enhance the nutraceutical quality of oyster mushrooms with potential benefits for human nutrition and sustainable agriculture.