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Vol. 14, Issue 1 (2025)

Production of the Anti-Parkinson’s drug L-DOPA by a novel bacterium Pseudomonas sp. DBF1

Author(s):
Lavanya D Tati, Rohini P Mule, Sainath H Kamble, Swati T Gurme and Shripad N Surwase
Abstract:
L-DOPA is an amino acid analogue utilized as a pharmacological agent for the management of Parkinson’s disease. This research investigated L-DOPA synthesis with the new bacterium Pseudomonas sp. DBF1. Bacteria that produce L-DOPA were isolated from garden soil samples for this study. The optimization studies for maximal L-DOPA synthesis encompass the influence of many physical parameters on L-DOPA yields, such as pH and temperature. The influence of nutritional parameters, such as carbon and nitrogen sources, was assessed by their incorporation into the medium. The influence of carbon sources includes diverse sugars such as glucose, sucrose, fructose, and lactose in a liquid media. The impacts of nitrogen sources encompass yeast extract, beef extract, peptone, and tryptone. The impacts of mineral salts involve manganese sulphate, copper sulphate, ferrous sulphate, zink sulphate, sodium chloride and potassium chloride. The effects of vitamins comprise cyanocobalamin, biotin, and ascorbic acid. The impact of amino acids includes L-phenylalanine, L-tryptophan, L-lysine, L-asparagine, and L-arginine. The impact of raw materials encompasses Glycine max (soybeans), Cicer arietinum (chickpeas), Cajanus cajan (pigeon peas), Lens culinaris (red lentils), and Phaseolus vulgaris (red kidney beans). The L-DOPA synthesizing strain Pseudomonas sp. DBF1, isolated from soil, exhibits a substantial production of 0.907 g/L when utilizing 1 g/L L-tyrosine at an optimal pH of 7, cultured at 30 °C for 24 hours, with peptone serving as the preferred nitrogen source. Copper sulphate and L-ascorbic acid markedly enhanced the synthesis of L-DOPA. Amino acids exhibited an inhibitory influence on L-DOPA synthesis. The synthesis of L-DOPA was analyzed utilizing GCMS and LCMS techniques. Pseudomonas species. DBF1 is an effective novel option for L-DOPA synthesis.
Pages: 70-84  |  93 Views  54 Downloads


The Pharma Innovation Journal
How to cite this article:
Lavanya D Tati, Rohini P Mule, Sainath H Kamble, Swati T Gurme, Shripad N Surwase. Production of the Anti-Parkinson’s drug L-DOPA by a novel bacterium Pseudomonas sp. DBF1. Pharma Innovation 2025;14(1):70-84.

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