Detection of citrus yellow mosaic virus in different species of citrus and in different tissues of infected sweet oranges by conventional PCR
Author(s):
Avinash CH, Rajasekharam T, Narasimha Rao S, Prasad TNVKV and Amaravathi Y
Abstract:
Citrus Yellow Mosaic Virus (CiYMV) is the etiologic agent of Citrus Yellow Mosaic Disease (CYMD), which has caused serious economic losses to Indian citrus industry. Detection of Citrus Yellow Mosaic Virus was carried out through PCR by using specific primers CiYMV F and CiYMV R primer set. The detection of CiYMV in different species of citrus showing bright amplification were observed at 726 bp. Similarly, the amplification was observed at 726 bp in different infected tissues of infected sweet orange tree viz., leaf, fruit rind, bark, twig, petiole, midrib leaf, feeder root, tender shoots, except young leaves through a conventional PCR.
How to cite this article:
Avinash CH, Rajasekharam T, Narasimha Rao S, Prasad TNVKV, Amaravathi Y. Detection of citrus yellow mosaic virus in different species of citrus and in different tissues of infected sweet oranges by conventional PCR. Pharma Innovation 2024;13(7):01-06.