Vol. 13, Issue 2 (2024)

Author(s):
Ravshankhon Makhmudov and Furkat Yunusov

Abstract:
A reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for determination and quantification those pharmaceutically active ingredients in tablets. The developed method was validated according to the ICH guidelines. Isocratic elution was performed with a mobile phase water: methanol: glacial acetic acid (70:25:5 v/v) mixture. The flow rate was 1.0 mL min-1 and UV detection was at 275 nm. The internal standards phenylephrine hydrochloride, paracetamol, caffeine and chlorpheniramine maleate retention times were within 4.07, 6.80, 9.70 and 26,46 minutes, respectively. The developed method was accurate, precise, robust and rapid enough. So it was applied successfully for the quality control assay of paracetamol, caffeine, phenylephrine hydrochloride and chlorpheniramine maleate in tablet dosage form.