Vol. 12, Issue 4 (2023)

Author(s):
Akanksha Gupta and AK Dang

Abstract:
To study the in vitro effect of LPS on PBMCs, apparently, healthy KF heifers were selected. To achieve this objective, the duration of exposure and different concentration of, LPS in PBMCs culture was used and IFNG levels were estimated. It was found that at 200 ng/ml dose rate of LPS IFNG levels were elevated with the increase in incubation hours. At 2, 4 hr incubation, the level of IFNG was high. Therefore, we can say with the increase in the duration of exposure of PBMCs IFNG levels increased.