Cytogenetic analysis using Fluorescence in situ Hybridization (FISH) of Acute Lymphoblastic Leukemia
Author(s):
Harshita Pandey and Dr. Pallavi Mittal
Abstract:
In pediatric acute lymphoblastic leukemia (ALL), chromosomal abnormalities are highly significant prognostic factors. In the present study peripheral blood or bone marrow samples of 60 children between age group of 1-17 diagnosed with ALL were analyzed for chromosomal abnormalities with fluorescence in situ hybridization (FISH) using locus specific probes to detect cryptic TEL/AML1 t (12:21) (p13-q22), BCR/ABL t (9;22) (q34;q11), TCF3- PBX1T(1:19)(q23;p13) and MLL rearrangement for 11q23. In the present study out of 60 cases 49 cases (81.66%) were normal and in 11 cases (18.33%) translocations were detected. Translocation t (12;21) was found in 7 cases and translocation t(9;22) was found in 4 cases. Any translocation was not detected in the age group of 1 to 9 years. 30.76% male cases and 13.04% female cases found to have translocations. Results showed that FISH improved the sensitivity and accurate identification of prognostic markers in pediatric ALL patients.
How to cite this article:
Harshita Pandey, Dr. Pallavi Mittal. Cytogenetic analysis using Fluorescence in situ Hybridization (FISH) of Acute Lymphoblastic Leukemia. Pharma Innovation 2023;12(2):3647-3650.