Vol. 12, Issue 2 (2023)

Author(s):
Manisha Shivran, Mahabeer Singh, Tejpal Bajaya, RP Ghasolia, Meera Choudhary and Sushila Choudhary

Abstract:
The clusterbean [Cyamopsis tetragonoloba (L.) Taub.] is belong to the Fabaceae family that derived from the Sanskrit word "Gauaahar," which means "livestock fodder. Wilt of clusterbean caused by Fusarium solani is a severe problem in clusterbean growing regions, the severity of the disease varies by region and it creates severe plant loss at the pre-emergence and post-emergence stages. Wilt of clusterbean caused by Fusarium solani is a major disease that causes severe seed yield losses among all fungal, bacterial and viral diseases. The pathogen was isolated from diseased root portions and purified using hyphal tip cut techniques before being identified as Fusarium solani based on morphology and colony characteristics. Pathogenicity was also shown under pot conditions using Koch's postulates procedures which also included seed, soil inoculation and seed cum soil inoculation. Seed cum soil inoculation method found most effective followed by soil inoculation method. The disease's first symptom appeared on the 10^th day after sowing and characteristic wilt symptoms such as yellowing plants, drooping leaves and browning and blackening of vascular tissues appeared within 30 days of sowing. The pathogen was re-isolated and the results were compared to the original cultures.