Vol. 12, Issue 2 (2023)

Author(s):
Saniya, P Pavan Kumar, Sarvamangala Cholin, Bapuraygouda Patil, Mahesh YS and AM Shirol

Abstract:
Induced mutagenesis using both chemical and physical mutagens is one of the noteworthy breeding tool to enhance variability in existing germplasm. Chemical mutagens such as DES, EMS, MMS and sodium azide have been commonly used to cause a mainstream of practical variations in several ornamental crops. For every mutation breeding experiment, determination of mutagenic sensitivity is an important step as it will vary with every species and varieties. In this investigation, semi-hard-wood cuttings of Nerium oleander pink and white genotypes were treated with ten different concentrations of DES ranging from 0.0% (untreated control) to 0.4% DES. The results shown steady and significant reduction in survival of cuttings with increase in dosage of DES. The probit curve analysis based on the survival percentage revealed the LD50 dosage of DES to be 0.3% for both nerium pink and white genotypes.