Vol. 12, Issue 2 (2023)

During the present study hematological and biochemical changes in blood of Rohu (Labeo rohita) in relation to Sex, Reproductive status and Environmental factors were analysed. Hematological parameters like haemoglobin (Hb), total erythrocyte count (TEC), packed cell volume (PCV), and Mean corpuscular haemoglobin concentration (MCHC) were found higher in males than in females, while the value for total leukocyte count mean corpuscular haemoglobin (MCV) and mean corpuscular haemoglobin (MCH) were found to be more in females than in males. TEC, TLC, Hb, PCV, and MCHC were found in increasing trend with increasing water temperature, while MCV and MCH were found to be in a decreasing trend with water temperature. Total protein content, albumin and globulin content were found to be more in males and were found in inverse correlation with temperature. Analysis of variance showed significant differences between the males and females for hematological and biochemical parameters except for Mean corpuscular volume and Mean corpuscular haemoglobin, the results indicated that the blood parameter levels from March to October were significantly different. The present study shows that that the haematological and biochemical parameters could be affected by sex, reproductive status and environmental conditions.