Vol. 12, Issue 2 (2023)

Author(s):
Vinay Kishor Tiwari, Yashica KA, Neethu CS and Sankar M

Abstract:
The aim of this study was to investigate the benzimidazole resistance (BR) reversal effect by combined treatment of thiabendazole and the specific P-gp inhibitor (calcium channel blocker), nifidepine on gastrointestinal nematodes. The representative samples known for BZ resistance (Rishikesh isolate) and susceptible (Kerala isolate) were used in the study. The samples were confirmed their susceptibility to BZ by egg hatch test (EHT) and allele specific PCR (AS-PCR). Furthermore, the eggs of these isolates were subjected to EHT using thiabendazole with or without nifidepine, a calcium channel blocker. The results revealed that nifidepine increased the toxicity of thiabendazole (TBZ), and the hatching of eggs with nifidepine being reduced significantly compared to without nifidepine. The P- gycoprotein (PGP) blocker (nifidepine) mediated reversal strategy might be enhance the efficacy of TBZ, a group of benzimidazole.