In-silico analysis of target genes of bta-miR-200a: A differentially expressed microRNA in lipopolysaccharide challenged peripheral blood mononuclear cells of crossbred and Vechur cattle
Author(s):
PD Divya, M Shynu, KK Jayavardhanan, R Uma, TV Aravindhakshan and G Radhika
Abstract:
MicroRNAs (miRNAs) are small, endogenous, non-coding RNAs which act as key regulators of a wide array of biological processes including cell proliferation, differentiation, apoptosis and mechanisms associated to cell signalling and immune responses. Through their effect on translational repression and gene silencing mechanisms, miRNAs play crucial role in post-transcriptional regulation of gene expression. Recent miRNA research works also reported that altered expression of miRNAs greatly influences the major cell signalling and immune related mechanisms. In the present study, target genes of the microRNA (miRNA), bta-miR-200a whose expression was found to be down regulated in lipopolysaccharide (LPS) stimulated peripheral blood mononuclear cells (PBMCs) of Vechur cattle with respect to crossbred cattle were predicted followed by gene ontology and pathway analysis of predicted target genes using various online bioinformatics tools. The study revealed significant enrichment of target genes in many immune related GO terms as well as critical cell signalling and multiple immune associated mechanisms.
How to cite this article:
PD Divya, M Shynu, KK Jayavardhanan, R Uma, TV Aravindhakshan, G Radhika. In-silico analysis of target genes of bta-miR-200a: A differentially expressed microRNA in lipopolysaccharide challenged peripheral blood mononuclear cells of crossbred and Vechur cattle. Pharma Innovation 2023;12(1):3066-3069.