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Vol. 12, Issue 1 (2023)

To develop an efficient and reproducible protocol for plant regeneration in cultivated and wild species of cluster bean

Author(s):
Puja Rani and Kuldeep Kumar
Abstract:
In the present research work plant regeneration studies were done in cultivated and wild species of cluster bean (Cyamopsis spp.).Three genotypes viz., HG2-20, HG563 and C. serrata were used for present investigation. For direct shoot regeneration C. tetragonoloba cultivars showed best response on MS medium supplemented with TDZ (5µM) as compared to wild species C. serrata. Maximum shoot induction response in immature cotyledon explants was observed (73%) on MS medium supplemented with TDZ (5µM) in cv. Minimum days to shoot induction (25 days) from immature cotyledons was observed on MS medium supplemented with TDZ (5µM) in C. tetragonoloba cv. HG 563. Maximum regeneration response from immature embryo axes explant was observed (97.7%) on MS medium supplemented with TDZ (5µM) in C. tetragonoloba cv. The best shoot regeneration response from cotyledonary node explants was observed (99.66%) on MS medium containing TDZ (5µM) in C. tetragonoloba cv. Out of three genotypes, highest callus induction response (82.80%) was observed on MS medium supplemented with TDZ (5 µM) in cultivar HG 563 and minimum days to callus induction (Sixteen days) was also observed on the same medium in all genotypes.MS basal supplemented with TDZ (5 µM) & NAA (2 mg/l) was observed as best medium for shoot induction and multiple shoot formation in all explants of three genotypes. Among all genotypes of C. serrata Shinz. was observed as best genotype for multiple shoot formation. Among all explants, Cotyledonary node was best explant for plant regeneration.
Pages: 2221-2232  |  310 Views  169 Downloads


The Pharma Innovation Journal
How to cite this article:
Puja Rani, Kuldeep Kumar. To develop an efficient and reproducible protocol for plant regeneration in cultivated and wild species of cluster bean. Pharma Innovation 2023;12(1):2221-2232.

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