Vol. 12, Issue 1 (2023)

Author(s):
Saniya, P Pavan Kumar, Sarvamangala Cholin, Bapuraygouda Patil, Mahesh YS and AM Shirol

Abstract:
Induced mutagenesis using both chemical and physical mutagens is one of the significant breeding tool to enhance variability in existing germplasm. Chemical mutagens such as EMS, DES, MMS and sodium azide have been widely used to cause a mainstream of practical variations in several flower crops. For every mutation breeding experiment, determination of mutagenic sensitivity is an important footstep as it will vary with every species and even among varieties. In this study, semi-hard-wood cuttings of Nerium oleander pink and white genotypes were treated with ten different concentrations of EMS ranging from 0.0% (untreated control) to 0.4% EMS. The results shown steady and significant reduction in survival of cuttings with increase in dosage of EMS. The probit curve analysis based on the survival percentage revealed the LD₅₀ dosage of EMS to be 0.4% and 0.3% for nerium pink and white genotypes respectively.