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Vol. 11, Issue 9 (2022)

Effect of different media on growth and cultural characterization of Alternaria alternata causing leaf spot of chilli

Author(s):
Shailja Goswami and Shubham Mishra
Abstract:
Alternaria is known for its most noticeable feature having concentric rings in the centre of the spots on leaves. In this present investigation, the morphological and cultural Charactization of the fungus was identified by performing the isolation of the pathogen from the diseased samples of the chilli leaves and establishing it’s identification through the pathogenicity test by spore suspension method and the results revealed that the identifying fungus showing the symptoms start appearing after seven to nine days of inoculation with small, circular brown to black spots surrounded by yellow hallow on chilli leaves. In severe cases, concentric rings appeared in the centre of the spots, the fungus was identified as Alternaria alternata. The radial growth pattern of Alternaria were observed on different temperatures, pH and humidity levels as well as also observed on different synthetic and non – synthetic media and the present study identified that the maximum radial growth of the fungus was observed at 30 °C (71 mm) temperature followed by 25 °C (69.5 mm) and the minimum growth was observed at 15 °C (26.5 mm) temperature. For the maximum growth of the fungus, the best pH observed was 6.5 (68.5 mm) followed by 6.0 (61 mm) and the minimum growth was observed at pH 4.0 (47 mm).The best relative humidity on which the pathogen exhibits maximum radial growth was 80 per cent (78.16 mm) followed by 90 per cent (74.66 mm) and the minimum growth was observed at 60 per cent (68.33 mm).The best synthetic media on which the maximum radial growth was observed is Richard’s agar (86.83 mm) and the best non-synthetic media on which the maximum radial growth was observed is Oatmeal agar media (75.5 mm). Thus by knowing the best temperature, pH and humidity levels as well as getting the best media other than PDA for the maximum growth of Alternaria alternata.
Pages: 56-64  |  689 Views  257 Downloads


The Pharma Innovation Journal
How to cite this article:
Shailja Goswami, Shubham Mishra. Effect of different media on growth and cultural characterization of Alternaria alternata causing leaf spot of chilli. Pharma Innovation 2022;11(9):56-64.

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