Vol. 11, Issue 8 (2022)

Author(s):
S Chandraprakash, R Rubini, VV Sangeetha Jebalin, M Rohini, S Shabaanaa Parwin and R Monicaa

Abstract:
Macrophomina phaseolina isolate NKL1 was isolated from the infected greengram roots showing typical symptoms of dry root rot collected from the crop cafeteria, PGP College of Agricultural Sciences, Namakkal. Six different solid media were tested for growth of pathogens, with maximum radial growth of mycelium (topography) on potato dextrose agar (9 cm). Among the two bio-control agents, Trichoderma viride and Bacillus subtilis, Trichoderma viride shows the maximum percent inhibition of mycelial growth (72.96%) and strong antagonistic activity against Macrophomina phaseolina under in vitro conditions. Among the various botanicals (Neem leaf extract, Pungam leaf extract, and garlic extract) used against Macrophomina phaseolina NKL1 at different concentrations (5%, 10%, and 15%), garlic extract with 10% and 15% concentration shows 100% inhibition of mycelial growth of Macrophomina phaseolina isolate NKL1.