Vol. 11, Issue 7 (2022)

Author(s):
Sakthi AR, Pavithra H, Gnanam R, Raveendran M and Sudha M

Abstract:
Green gram is an important legume crop grown widely in different parts of the Asian countries. The need for food grains increases due to the growing world’s population. The recalcitrance nature of green gram for in vitro regeneration and differential response of explants, cultivars hampers the genetic improvement of the crop. Therefore in vitro regeneration protocol is essential for improvement of each cultivar/variety. For the present study, explants of cotyledon, hypocotyls and leaf of Vamban 2 variety derived from five and seven day old in vitro grown seedlings were used. The excised explants were inoculated in different combinations of BAP and NAA in MS basal medium. The response of regeneration varies among the explants and also the age of the seedlings used. The inclusion of hormone NAA in two different concentrations and in combination with BAP produced hard calli with roots. Cotyledon and leaf explants produced multiple shoots in the shoot induction media supplemented with BAP (0.5 mg/L) and the elongated shoots were rooted in ½ MS media with 1 mg/L IBA. The protocol established can be used for improvement of cultivar through genetic engineering or genome editing for important traits.