Vol. 11, Issue 7 (2022)

Author(s):
A Khound Sharmah, D Sharmah and P Sen

Abstract:
The genetic variation in Piper longum germplasm collected from North East states, India was investigated using Random Amplified Polymorphic DNA (RAPD) markers. A total of sixteen P. longum germplasms were considered for RAPD analysis and yielded appreciable amount of good quality DNA (A₂₆₀/A₂₈₀=1.8). The result indicated that the differential polymorphism in 16 different germplasm showing variation in percentage of polymorphism (25.01 to 60.00) in twenty decamer random primers. The maximum level of polymorphism was observed in OPB19 (60.00%), followed by OPB 1 (57.14%), OPA08 (50.5%), OPB02 (50.0%) OPB20 (50.0%) and OPA 11 (50.0%). Altogether one hundred and five (105) bands were amplified out of which forty one (41) bands were polymorphic. The UPGMA cluster analysis of RAPD banding pattern for correlation coefficient of similarity matrix showed that similarity value ranged from 0.587 to 0.862 indicating moderate genetic diversity among the germplasm studied. The dendrogram grouping pattern revealed that the germplasm formed four main clusters, cluster I (PLJ-18, PLJ-19, PLJ-03 and PLJ-20), Cluster II (PLJ-28, PLJ-29, PLJ-30 and PLJ-32), Cluster III (PLJ-09, PLJ-01, PLJ-22, PLJ-17 and PLJ-11) and cluster IV (PLJ-16 & PLJ-10). The check variety “Viswam” a collection from Kerala was considered as out group due to the originality of the variety.