Vol. 11, Issue 6 (2022)

The present experiment was carried out to study the effect oral administration of mustard oil in insulin treated diabetic dogs. Dogs visiting Small Animal Clinics, were taken for the study. Six healthy dogs were treated as group-I. Six diabetic dogs treated with insulin were treated as group-II. Six diabetic dogs treated with insulin along with oral administration of mustard oil were treated as group-III. Dogs in group-II and III were treated daily with insulin at the dose rate of 0.2 to 1 U/kg body weight by subcutaneous route once. Group-III dogs were administered orally with mustard oil at the dose rate of 0.25g per kg body weight. The study was carried out for a period of sixty days. Blood samples collected were used for haematological and biochemical assays. There was no significant change in the hemogram and leukogram values, except platelet count which was increased significantly in treatment groups. There was a reduction in blood glucose, HbA1c level and increase in body weight in mustard oil treated diabetic dogs. A significant increase in serum total cholesterol and HDL-cholesterol level were noticed in mustard oil treated groups. There was no significant difference in the levels of total protein, albumin and globulin among the groups. Administration of mustard oil significantly reduced the serum BUN and creatinine level in diabetic dogs treated with insulin and mustard oil. This indicates the protective role of the mustard oil in kidney function, due to the anti-hyperglycemic activity. There was no significant change in the serum electrolytes values except serum calcium level which was a significant reduced in mustard oil treated group, which may be due to precipitation of calcium by excess fatty acids present. The findings suggest that mustard oil supplementation can improve the glycemic control in diabetic dogs which may be due to mono and polyunsaturated fatty acids present.