Abstract:In this study,
Forty eight anestrus postpartum Murrah buffaloes (over 90 days) were randomly selected and equally divided into four groups. Animals were subjected to treatment with CIDR implant+PGF2 α (Lutalyse)+ PMSG, Crestar + PGF2 α (Lutalyse)+PMSG, Intravaginal progesterone impregnated sponge implant+PGF2 α (Lutalyse)+ PMSG and Intravaginal progesterone impregnated sponge implant with CMC+PGF2 α (Lutalyse)+ PMSG as group I, II, III and IV respectively. Biochemical profile of serum were analysed after collection of blood on day 0, 3
rd, 6
th and 9
th of hormonal treatment. The level of glucose (61.05±2.33 to 70.53±2.40 mg/dl), total protein (7.15±0.17 to 7.90±0.28 g/dl), cholesterol (95.99±4.48 to 116.48±5.88 mg/dl), calcium (10.12±0.27 to 10.37±0.11 mg/dl) were within the normal range in all groups. However, the level of phosphorus (3.26±0.08 to 3.59±0.09 mg/dl) was lower compared to reference value (4.39-7.85

) in all groups, may be one of contributing factor for postpartum anestus in buffaloes.