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Vol. 11, Issue 5 (2022)

Vitamin B12 enriched milk based nutraceutical production using Propionibacterium freudenreichii

Author(s):
Harish Kumar Kurmindla, Suvarna Veerappa Chavannavar, Sarvani Bharathula, Abhishek Umesh Rayasandhra and Banu Deshpande
Abstract:
A Propionibacterium freudenreichii strain that was well characterized for its probiotic characteristics like pH, bile salt tolerances, sensitivity to commonly used antibiotics was employed as a starter culture to develop vitamin B12 enriched nutraceutical product. The experiment was designed using five treatments with starter inocula as home-made curd (T1), Propionibacterium freudenreichii (T2), Lactobacillus acidophilus (T3), Propionibacterium freudenreichii + Lactobacillus acidophilus (T4) and yoghurt (T5). Milk was fermented at 37 °C for 8 h using these starter cultures. The developed product after fermentation was analyzed for vitamin B12 content, physico-chemical properties and was subjected to sensory evaluation. Treatment T2 with inoculum Propionibacterium freudenreichii was found to produce the significantly highest vitamin B12 (10.37 µg 100mL-1) along with the best score (8.15) of acceptance for sensory evaluation parameters. The second best sensory score was given to treatment T5 (7.9) while the second highest vitamin B12 ­production was found in treatment T4 (8.34 µg 100mL-1). One serving of the product (10.37µg100mL-1) provides vitamin B12 more than recommended daily allowance (2.4 µg).
Pages: 46-50  |  522 Views  117 Downloads


The Pharma Innovation Journal
How to cite this article:
Harish Kumar Kurmindla, Suvarna Veerappa Chavannavar, Sarvani Bharathula, Abhishek Umesh Rayasandhra, Banu Deshpande. Vitamin B12 enriched milk based nutraceutical production using Propionibacterium freudenreichii. Pharma Innovation 2022;11(5):46-50.

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