Vol. 11, Issue 5 (2022)

Author(s):
Gawande SM, Ghuge SB, Kalpande HV and Rathod ST

Abstract:
In present investigation, the seeds of safflower variety PBNS-12 were treated with different doses of gamma rays viz., 100 Gy, 200 Gy, 300 Gy, 400 Gy, 500 Gy and different concentrations of EMS viz., 0.1%, 0.2%, 0.3%, 0.4% and their combination treatments. The plant survival percentage was greatly affected by mutagenic treatments of gamma rays and EMS which showed a negative dose dependent relationship in PBNS-12 variety. Based on mortality percentages and probit values, the expected LD₅₀ value was computed. The LD₅₀ value for PBNS-12 was fixed at 299.5 Gy for gamma rays and 0.25% for EMS. Gamma rays caused the greatest decline in plant survival percentage, followed by combination and EMS treatments. Gamma rays exhibited significant reduction in plant survival percentage (1%) than EMS (24.45%) in PBNS-12 variety of safflower. It is concluded that both the mutagens are effective to produce significant variations in safflower which can be further explored for mutation mapping.