Effect of different cultural media on growth of different isolates of Sclerotium rolfsii Sacc. causing stem rot of Indian bean
Author(s):
Ravikumar G Vaniya, Pushpendra Singh and AJ Deshmukh
Abstract:
Sclerotium rolfsii Sacc. is one of the most important soil-borne plant pathogen which causes severe loss at the time of seedling development. It also causes stem rot or collar rot in several crops and wild plants. In this present investigation five different cultural solid and liquid media viz., Potato dextrose agar and broth, Host extract agar and broth (Indian bean), Czapek’s agar and broth, Richard’s agar and broth and Sabouraud’s agar and broth were analyzed for in vitro mycelial growth and dry mycelial weight of different eight isolates of S. rolfsii collected from different location. The cultural characteristics indicated that the excellent mycelial growth and dry mycelial weight were observed on different semi-synthetic media viz., Potato dextrose agar and broth followed by Host leaf extract agar medium and broth (Indian bean), Czapek’s dextrose agar medium and broth, Richard’s Agar medium and broth. Sabouraud’s Agar medium and broth recorded comparatively less growth and dry mycelial weight of different eight isolates of S. rolfsii.
How to cite this article:
Ravikumar G Vaniya, Pushpendra Singh, AJ Deshmukh. Effect of different cultural media on growth of different isolates of Sclerotium rolfsii Sacc. causing stem rot of Indian bean. Pharma Innovation 2022;11(4):14-19.