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Vol. 11, Issue 3 (2022)

Conventional and molecular identification of bacterial endophyte: Gluconacetobacter diazotrophicus from different Sugarcane cultivars

Author(s):
Belliraj Natarajan
Abstract:
The bacterial endophytes are plant-associated and they can survive in the internal tissues of the plant without harming the host plant. The endophytes play a vital role in plant growth promotion as they directly or indirectly promote plant growth. The genus Gluconacetobacter encompasses different species which are immensely used in agricultural and industrial sectors. This study aims to identify the presence of Gluconacetobacter diazotrophicus and in sugarcane crop considering their potential biotechnological applications. Eighteen isolates of Gluconacetobacter diazotrophicus were obtained from the root, bud, and leaf portions of sugarcane varieties (CoC 92016, Co 86032, Co 86032, Co 8608 and Co 86034) collected from Thondamuthur, Sathyamangalam, Aaapakodal of Coimbatore and Erode districts, Sugarcane Breeding Institute, Coimbatore, and Eastern Block of Tamil Nadu Agricultural University, Coimbatore. The isolates are identified biochemical evaluations, and molecular and phylogenetic analyses. These isolates were characterized and confirmed by comparing with the standard type strains PAL 5 and L5. All the isolates produced orange yellow colonies on LGI and Acetic LGI agar plates and chocolate brown colonies on Potato agar plates. Based on the colony morphology and biochemical analysis best four isolates are selected for the 16S r RNA analysis. The phylogenetic characterization of 16S r RNA gene sequencing analysis of isolates showes very distinct to other closely related genera of Gluconacetobacter diazotrophicus. The isolates have ecological significance and influence the advance research towards the evaluation of plant-soil interactions in sugarcane eco systems.
Pages: 07-11  |  567 Views  155 Downloads


The Pharma Innovation Journal
How to cite this article:
Belliraj Natarajan. Conventional and molecular identification of bacterial endophyte: Gluconacetobacter diazotrophicus from different Sugarcane cultivars. Pharma Innovation 2022;11(3):07-11.

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