Vol. 11, Issue 2 (2022)

Author(s):
Pinki Singh, Raju Prasad and Shamshun Nehar

Abstract:
A great source of information for variety of phytochemicals present in a medicinal plant which can be developed as drug with the precise selectivity. The present study was aimed to evaluate qualitative phytochemical screening of methanolic extract of Achyranthes aspera followed by Thin Layer Chromatography (TLC) / High Performance Thin Layer Chromatography (HPTLC) analysis. TLC/HPTLC was performed on pre-coated silica gel G 60 F254 plates using mobile-phase containing Toluene: Chloroform: Methanol in the ratio of 3:6:1 respectively. The plates were developed in saturated mode of twin trough chamber at room temperature. The bands were observed and photographed in ultraviolet light at 254 nm and 366 nm. Densitometer scanning of Achyranthes aspera revealed 6 major peaks at 366 nm with sample volume 10 μl at 10 mg/ml concentration in methanol. The screening confirms the presence of various phytoconstituents like alkaloids, flavonoids, terpenoids, saponins, carbohydrates, glycosides etc. The current study may give a scientific basis to the plant already has been used for traditional uses to treat various ailments and metabolic disorders which have not been evaluated.